Efecto de las células oviductales y del cumulus oophorus sobre diferentes parámetros biológicos relacionados con la fecundación in vitro en la especie porcina.
- Romar Andrés, Raquel
- María Pilar Coy Fuster Director
- Salvador Ruiz López Director
Universidade de defensa: Universidad de Murcia
Fecha de defensa: 24 de xaneiro de 2001
- Ronald H. F. Hunter Presidente/a
- Emilio Gómez de la Concha Secretario
- María Teresa Paramio Nieto Vogal
- Detlef Rath Vogal
- Raúl Sánchez Vogal
Tipo: Tese
Resumo
The objective of this experimental work was to study the effect of porcine epithelial oviductal cells and cumulus cells on several parameters related to in vitro fertilization in pigs. Studied variables were rate of penetration, mean number of sperm cells per penetrated oocyte, rate of male pronuclear formation, cleavage rate at 48h postinsemination, blastocyst formation rate, mean number of cells per blastocyst, resistance of zona pellucida to pronase digestion and density and distribution of cortical granules in the different experimental groups.