Efecto de las células oviductales y del cumulus oophorus sobre diferentes parámetros biológicos relacionados con la fecundación in vitro en la especie porcina.
- Romar Andrés, Raquel
- María Pilar Coy Fuster Directeur/trice
- Salvador Ruiz López Directeur/trice
Université de défendre: Universidad de Murcia
Fecha de defensa: 24 janvier 2008
- Ronald H. F. Hunter President
- Emilio Gómez de la Concha Secrétaire
- María Teresa Paramio Nieto Rapporteur
- Detlef Rath Rapporteur
- Raúl Sánchez Rapporteur
Type: Thèses
Résumé
The objective of this experimental work was to study the effect of porcine epithelial oviductal cells and cumulus cells on several parameters related to in vitro fertilization in pigs. Studied variables were rate of penetration, mean number of sperm cells per penetrated oocyte, rate of male pronuclear formation, cleavage rate at 48h postinsemination, blastocyst formation rate, mean number of cells per blastocyst, resistance of zona pellucida to pronase digestion and density and distribution of cortical granules in the different experimental groups.