Publications by the researcher in collaboration with José Luis García López (12)

2007

  1. Production of a thermoresistant alpha-galactosidase from Thermus sp. strain T2 for food processing

    Food Biotechnology, Vol. 21, Núm. 1, pp. 91-103

2004

  1. A simple strategy for the purification of large thermophilic proteins overexpressed in mesophilic microorganisms: Application to multimeric enzymes from Thermus sp. strain T2 expressed in Escherichia coli

    Biotechnology Progress, Vol. 20, Núm. 5, pp. 1507-1511

  2. Selective and mild adsorption of large proteins on lowly activated immobilized metal ion affinity chromatography matrices: Purification of multimeric thermophilic enzymes overexpressed in Escherichia coli

    Journal of Chromatography A, Vol. 1055, Núm. 1-2, pp. 93-98

  3. Stabilization of a Multimeric β-Galactosidase from Thermus sp. Strain T2 by Immobilization on Novel Heterofunctional Epoxy Supports Plus Aldehyde-Dextran Cross-Linking

    Biotechnology Progress, Vol. 20, Núm. 1, pp. 388-392

2003

  1. One-step purification, covalent immobilization, and additional stabilization of a thermophilic poly-his-tagged β-galactosidase from Thermus sp. strain T2 by using novel heterofunctional chelate - Epoxy sepabeads

    Biomacromolecules, Vol. 4, Núm. 1, pp. 107-113

  2. Overproduction of Thermus sp. strain T2 β-galactosidase in Escherichia coli and preparation by using tailor-made metal chelate supports

    Applied and Environmental Microbiology, Vol. 69, Núm. 4, pp. 1967-1972

  3. Reversible immobilization of a thermophilic β-galactosidase via ionic adsorption on PEI-coated Sepabeads

    Enzyme and Microbial Technology, Vol. 32, Núm. 3-4, pp. 369-374

  4. The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition: Complete hydrolysis of lactose in dairy products

    Enzyme and Microbial Technology, Vol. 33, Núm. 2-3, pp. 199-205

2002

  1. Conformational selection of glycomimetics at enzyme catalytic sites: Experimental demonstration of the binding of distinct high-energy distorted conformations of C-, S-, and O-glycosides by E. Coli β-galactosidases

    Journal of the American Chemical Society, Vol. 124, Núm. 17, pp. 4804-4810

2001

  1. Affinity chromatography of polyhistidine tagged enzymes - New dextran-coated immobilized metal ion affinity chromatography matrices for prevention of undesired multipoint adsorptions

    Journal of Chromatography A, Vol. 915, Núm. 1-2, pp. 97-106

1999

  1. Escherichia coli β-galactosidase recognizes a high-energy conformation of C-lactose, a nonhydrolyzable substrate analogue. NMR and modeling studies of the molecular complex

    Chemtracts

1998

  1. Structure of the β-galactosidase gene from Thermus sp. Strain T2: Expression in Escherichia coli and purification in a single step of an active fusion protein

    Applied and Environmental Microbiology, Vol. 64, Núm. 6, pp. 2187-2191