Detección de ADN bovino y ovino en piensos comerciales mediante una técnica de PCR en tiempo real
- Nicolette Pegels Rojas 1
- Isabel González Alonso 1
- Rosario Martín de Santos 1
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1
Universidad Complutense de Madrid
info
ISSN: 1988-2688
Datum der Publikation: 2009
Ausgabe: 3
Nummer: 2
Seiten: 176-183
Art: Artikel
Andere Publikationen in: Revista complutense de ciencias veterinarias
Zusammenfassung
A polymerase chain reaction (PCR) assay using TaqMan probes has been developed for detection of bovine and ovine DNA commercial feedstuffs. The real-time PCR method combines the use of bovine and ovine-specific primers that amplify DNA fragments of 84 bp on the mitochondrial 12S ribosomal RNA gene and 88 bp on the mitochondrial D-loop region, respectively, together with universal primers that amplify a 141 bp DNA fragment on the nuclear 18S ribosomal RNA gene from eukaryotic organisms. The PCR method developed in this assay was able to identify as low as 0.1% bovine and ovine content of meat and bone meals in the feed samples analyzed.