Detección de ADN bovino y ovino en piensos comerciales mediante una técnica de PCR en tiempo real

  1. Nicolette Pegels Rojas 1
  2. Isabel González Alonso 1
  3. Rosario Martín de Santos 1
  1. 1 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

Aldizkaria:
Revista complutense de ciencias veterinarias

ISSN: 1988-2688

Argitalpen urtea: 2009

Alea: 3

Zenbakia: 2

Orrialdeak: 176-183

Mota: Artikulua

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Beste argitalpen batzuk: Revista complutense de ciencias veterinarias

Laburpena

A polymerase chain reaction (PCR) assay using TaqMan probes has been developed for detection of bovine and ovine DNA commercial feedstuffs. The real-time PCR method combines the use of bovine and ovine-specific primers that amplify DNA fragments of 84 bp on the mitochondrial 12S ribosomal RNA gene and 88 bp on the mitochondrial D-loop region, respectively, together with universal primers that amplify a 141 bp DNA fragment on the nuclear 18S ribosomal RNA gene from eukaryotic organisms. The PCR method developed in this assay was able to identify as low as 0.1% bovine and ovine content of meat and bone meals in the feed samples analyzed.

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