Polymerase chain reaction detection of rabbit DNA in food and animal feed.

  1. Martín, Irene
  2. García Lacarra, Teresa
  3. Fajardo Martín, Violeta
  4. Rojas Diéguez, María
  5. Pegels Rojas, Nicolette
  6. Hernández Cruza, Pablo E.
  7. González Alonso, Isabel
  8. Martín de Santos, María del Rosario
Aldizkaria:
World Rabbit Science

ISSN: 1989-8886,  1257-5011

Argitalpen urtea: 2009

Alea: 17

Zenbakia: 1

Orrialdeak: 27-34

Mota: Artikulua

DOI: 10.4995/WRS.2009.667 DIALNET GOOGLE SCHOLAR lock_openSarbide irekia editor

Beste argitalpen batzuk: World Rabbit Science

Laburpena

A polymerase chain reaction (PCR) based on oligonucleotide primers targeting the mitochondrial 12S rRNA gene was developed for the specific identification of rabbit DNA (Oryctolagus cuniculus) in food and feedstuffs. The specificity of the primers was verified by PCR analysis of DNA from 32 non-target species including mammals, birds, fish, and plant species. Analysis of experimental mixtures demonstrated the presence of rabbit-derived materials in the range of 0.1-100%. Prolonged heat treatment (up to 133ºC for 20 min at 300 kPa) applied to rabbit muscle/oats binary mixtures did not affect the performance of the method, which could therefore be said to be very useful for the accurate identification of rabbit materials in products submitted to denaturing technologies when other methods are not suitable. 

Erreferentzia bibliografikoak

  • Arslan A., Ilhak I., Calicioglu M., Karahan M. 2005. Identification of meats using random amplified polymorphic DNA (RAPD) tecnique. Journal of Muscle Foods, 16: 37-45.
  • Colgan S., O’brien L., Maher M., Shilton N., Mcdonnell K., Ward S. 2001. Development of a DNA-based assay for species identification in meat and bone meal. Food Res. Int., 34: 409-414.
  • Chisholm J., Conyers C., Booth C., Lawley W., Hird H. 2005. The detection of horse and donkey using real-time PCR. Meat Sci., 70: 727-732.
  • Dalmasso A., Fontanella E., Piatti P., Civera T., Rosatti S., Bottero M.T. 2004. A multiplex PCR assay for the identification of animal species in feedstuffs. Molecular and Cellular Probes, 18: 81-87.
  • Di Pinto A., Forte V.T., Conversano M.C., Tantillo G.M. 2005. Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources. Food Control, 16: 391394.
  • EC Council. 2001. Regulation laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies. No. 999/2001/EC, 22 May 2001. Off. J. Eur. Comm., 31 May 2001, L147.
  • EC Council. 2002. Regulation laying down health rules concerning animal by-products not intended for human consumption. No. 1774/2002/EC, 3 October 2002. Off. J. Eur. Comm., 10 October 2002, L 273, 1-95.
  • Fajardo V., González I., López-Calleja I., Martín I., Hernández P.E., García T., Martín R. 2006. PCR-RFLP Authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries) and goat (Capra hircus). J. Agric. Food Chem., 54: 1144-1150.
  • Fajardo V., González I., López-Calleja I., Martín I., Rojas M., Pavón M.A., Hernández P.E., García T., Martín R. 2007. Analysis of mitochondrial DNA for authentication of meats from chamois (Rupicapra rupicapra), pyrenean ibex (Capra pyrenaica), and mouflon (Ovis ammon) by polymerase chain reaction-restriction fragment length polymorphism. J. AOAC Int., 90: 179-186.
  • Hird H., Chisholm J., Sánchez A., Hernández M., Goodier R., Schneede K., Boltz C., Popping B. 2006. Effect of heat and pressure processing on DNA fragmentation and implications for the detection of meat using a real-time polymerase chain reaction. Food Additiv. Contamin., 23: 645-650.
  • Herman B.L., 2001. Determination of the animal origin of raw food by species-specific PCR. J. Dairy Res., 68: 429-436.
  • Hunt D.J., Parkes H.C., Lumley I.D., 1997. Identification of the species of origin of raw and cooked meat products using oligonucleotide. Food Chem., 60: 437-442.
  • Kusama T., Nomuta T., Kadowaki K. 2004. Development of primers for detection of meat and bone meal in ruminant feed and identification of the animal origin. J. Food Prot., 67: 1289-1292.
  • Martín I., García T., Fajardo V., López-Calleja I., Hernández P.E., González I., Martín R. 2007a. Species-specific PCR for the identification of ruminant species in feedstuffs. Meat Sci., 75: 120127.
  • Martín I., García T., Fajardo V., Rojas M., Hernández P.E., González I., Martín R. 2007b. Technical note: Detection of cat, dog and rat/mouse tissues in food and animal feed using species-specific polymerase chain reaction. J. Anim. Sci., 85: 2734-2739.
  • Martín I., García T., Fajardo V., Rojas M., Hernández P.E., González I., Martín. R. 2008. Real-Time PCR for quantitative detection of bovine tissues in food and feed. J. Food Prot., 71: 564-572.
  • Matsunaga T., Chikuni K., Tanabe R., Muroya S., Nakai H., Shibata K., Yamada J., Shinmura Y. 1999. A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Sci., 51: 143-148.
  • Partis L., Ctroan D., Guo Z., Clark R., Coldham T., Murby J. 2000. Evaluation of a DNA fingerprinting method for determining the species origin of meats. Meat Sci., 54: 369-376.
  • Pascal G., Mahé S. 2001. Identity, traceability, acceptability and substantial equivalence o food. Cellular and Molecular Biology, 47: 1329-1342.
  • Rodríguez M.A., García T., González I., Asensio L., Hernández P.E., Martín R. 2003. Identification of goose, mule duck, chicken, turkey, and swine in foie-gras by species-specific polymerase chain reaction. J. Agric. Food Chem., 51: 1524-1529.
  • Rodríguez M.A., García T., González I., Asensio L., Hernández P.E., Martín R. 2004. PCR identification of beef, sheep, goat and pork in raw and heat treated meat mixtures. J. Food Prot., 67: 172-177.
  • Schlumpberger T. 2004. DNA test identifies animal species in food products. Food Technology Feature, 58: 44-49.
  • Teletchea F., Maudet C., Hänni C. 2005. Food and forensic molecular identification: update and challenges. Trends in Biotechnology, 23: 359-366.
  • Walker J.A., Hughes D.A., Hedges D.J., Anders B.A., Laberde M.E., Shewale J., Sinha S.K., Batzer M.A. 2004. Quantitative PCR for DNA identification based on genome-specific interspersed repetitive elements. Genomics, 83: 518-527
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